Recombinant Human aFGF

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詳細介紹

品牌	absin	貨號	abs00875
規格	10ug	供貨周期	一周

概述
種屬	Human
形態	Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
來源	Escherichia coli.
氨基酸序列	MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
內毒素水平	Less than 1 EU/µg of Recombinant HumanaFGF as determined by LAL method.
活性	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg in the presence of 10 μg/ml of heparin.
Accession #	P05230
預期分子量	Approximay 16.0 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids.

性能
溶解方法	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
保存方法	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
純度	> 95 % by SDS-PAGE and HPLC analyses.
Gene IDs	2246

參考文獻
1. Dionne CA, Crumley G, Bellot F, et al. 1990. EMBO J. 9:2685-92. 2. Jaye M, Howk R, Burgess W, et al. 1986. Science. 233:541-5. 3. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6. 4. Gospodarowicz D. 1974. Nature. 249:123-7. 5. Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700. 6. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7. 7. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49. 8. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57. 9. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.

參考文獻

1. Dionne CA, Crumley G, Bellot F, et al. 1990. EMBO J. 9:2685-92.

2. Jaye M, Howk R, Burgess W, et al. 1986. Science. 233:541-5.

3. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.

4. Gospodarowicz D. 1974. Nature. 249:123-7.

5. Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700.

6. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.

7. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.

8. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.

9. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.

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